Journal: PLoS ONE

Article Title: Expression of mycobacterium tuberculosis induced SOCS3 and STAT3 and the implications on innate immunity in TB patients vs healthy contacts in high TB/HIV endemic setting: A cross-sectional analytical study

doi: 10.1371/journal.pone.0263624

Figure Lengend Snippet: A1: Shows that SOCS3 increased significantly post stimulation in TB patients, Median (IQR) 0.0 (0.0–0.0) in pre-stimulation to 0.7 (0.1–1.75), p value = <0.0001. A2: Shows significant rise in SOCS3 in healthy contacts post exposure to heat killed MTB, 1.4 (0.4–6.9) in pre-stimulation to 6.4 (1.65–21.95) post- stimulation, p value = 0.003. B1: Shows increased expression of STATS3 post stimulation in TB patients, 0.0 (0.0–0.0) in pre-stimulation to 408.6 (0.002–2,220,630) post-stimulation, p value = <0.0001. B2: Shows the shift in STAT3 expression following stimulation in healthy contacts, 0.1 (0.0–0.1) in pre-stimulation to 93,234 (6,684–118,833) post-stimulation, p value <0.0001. Statistical significance was evaluated using Wilcoxon signed-rank test.

Article Snippet: Human primers SOCS3, STATS3 and GAPDH were acquired from Sino biological.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Expression of mycobacterium tuberculosis induced SOCS3 and STAT3 and the implications on innate immunity in TB patients vs healthy contacts in high TB/HIV endemic setting: A cross-sectional analytical study

doi: 10.1371/journal.pone.0263624

Figure Lengend Snippet: A: Shows statistically significant higher levels of SOCS3 in healthy contacts when compared to TB patients. Median (IQR) 0.0 (0.0–0.0) in TB patients vs 1.4 (0.4–6.9) in healthy contacts, p value = < 0.0001. B. Shows the hardly any expression of STAT3 pre-stimulation in both TB patients and healthy contacts. Statistical significance evaluated using Mann–Whitney test.

Article Snippet: Human primers SOCS3, STATS3 and GAPDH were acquired from Sino biological.

Techniques: Expressing, MANN-WHITNEY

Journal: PLoS ONE

Article Title: Expression of mycobacterium tuberculosis induced SOCS3 and STAT3 and the implications on innate immunity in TB patients vs healthy contacts in high TB/HIV endemic setting: A cross-sectional analytical study

doi: 10.1371/journal.pone.0263624

Figure Lengend Snippet: A : Shows significantly higher expression of SOCS3 in healthy contacts than TB patients post-stimulation. Median (IQR) 0.75 (0.175–1.8) in TB patients vs 6.4 (1.65–21.95) in healthy contacts, p value = < 0.0001. B : Shows no statistically difference in expression of in TB patients vs 93234 (966,847–118,833) in healthy contacts, p value = 0.0913. Statistical significance was evaluated using Mann–Whitney test.

Article Snippet: Human primers SOCS3, STATS3 and GAPDH were acquired from Sino biological.

Techniques: Expressing, MANN-WHITNEY

Journal: PLoS ONE

Article Title: Expression of mycobacterium tuberculosis induced SOCS3 and STAT3 and the implications on innate immunity in TB patients vs healthy contacts in high TB/HIV endemic setting: A cross-sectional analytical study

doi: 10.1371/journal.pone.0263624

Figure Lengend Snippet: HIV negative healthy contacts expressed significantly higher levels of SOCS3 in comparison to both HIV negative TB patients and TB/HIV patients, P values was < 0.0001 for individual evaluations. We found no statistically significant difference in the expression of SOCS3 between HIV positive healthy and HIV negative healthy contacts and both groups of TB patients.

Article Snippet: Human primers SOCS3, STATS3 and GAPDH were acquired from Sino biological.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Expression of mycobacterium tuberculosis induced SOCS3 and STAT3 and the implications on innate immunity in TB patients vs healthy contacts in high TB/HIV endemic setting: A cross-sectional analytical study

doi: 10.1371/journal.pone.0263624

Figure Lengend Snippet: A: Shows a negative correlation between post-stimulation expression SOCS3 and IL-6 in TB patients and B shows a positive and significant correlation between post-stimulation expression SOCS3 and IL-6. The relationship between SOCS3 and IL-6 was assessed using spearman’s rank correlation.

Article Snippet: Human primers SOCS3, STATS3 and GAPDH were acquired from Sino biological.

Techniques: Expressing

Journal: Pharmaceutical Biology

Article Title: Shenlian extract attenuates myocardial ischaemia-reperfusion injury via inhibiting M1 macrophage polarization by silencing miR-155

doi: 10.1080/13880209.2022.2117828

Figure Lengend Snippet: The effect of SL on SOCS3 expression. (A, B) SOCS3 gene expression and quantitative analysis by qRT-PCR. (C, D) SOCS3 proteins expression of and quantitative analysis in pMACs by western blotting. Results were presented as mean ± SD. * p < 0.05, ** p < 0.01 vs. control group, # p < 0.05, ## p < 0.01 vs. model group, n = 3.

Article Snippet: SOCS3 (Cat. No: A00274-2) antibody was purchased from BOSTER. iNOS (Cat. No: 18985-1-AP) antibody was obtained from Proteintech.

Techniques: Expressing, Gene Expression, Quantitative RT-PCR, Western Blot, Control

Journal: Transplantation

Article Title: Activated AXL Protects Against Hepatic Ischemia-reperfusion Injury by Upregulating SOCS-1 Expression

doi: 10.1097/TP.0000000000004156

Figure Lengend Snippet: Activated AXL inhibits the TLR/MYD88/NF-κB pathway by upregulating SOCS-1. A, Western blotting analysis of the expression of SOCS-1, SOCS-3, TLR4, MYD88, TRAF6, IκBα, and p-P65 in the rmGas6 treatment group and the control group. B, Western blotting used to detect the expression of SOCS-1, TLR4, MYD88, TRAF6, IκBα, and p-P65 in the R428 treatment group. C, Representative immunofluorescence pictures showing the expression of SOCS-1. For (A–C) each group n = 3–6. *** P < 0.001; ** P < 0.01; * P < 0.05. AXL, AXL receptor tyrosine kinase; DAPI, 4′,6-diamidino-2-phenylindole; IκBα, NF-κB inhibitor alpha; I/R, ischemia-reperfusion; MYD88, myeloid differentiation factor-88; NF-κB, nuclear factor kappa-B; ns, not significant; p-AXL, phosphorylation AXL; p-P65, phospho-NF-κB-p65; rmGas6, recombinant growth arrest-specific protein 6; SOCS, suppressor of cytokine signaling protein; TLR4, toll-like receptor 4; TRAF6, tumor necrosis factor receptor–associated factor 6.

Article Snippet: SOCS-3 , Zenbio (China) , 500694 , 1:1000.

Techniques: Western Blot, Expressing, Immunofluorescence, Recombinant